韭菜生产中施肥量、播量与产量的相关关系张桂海 王明耀 王学颖 黄志辉 王长浩 田庆武 张钊

通过播种量与施肥量的组合试验,寻求韭菜合理的播种量的区间与最佳的施肥量,得到韭菜生产种子的播量范围为2.38kg /667㎡～3.00kg/667㎡。     

小量纠偏法在履带甘蔗收获机中的应用

广西柳工机械股份有限公司某款履带式甘蔗收获机样机在直线行走测试过程中出现"跑偏"问题。为了解决该问题,本文采用了小量纠偏法来控制行走电比例阀输出电流进而控制变量马达的排量。该方法将该款机型直线行走误差控制在纠偏前误差的±25%,纠偏效果良好,满足客户需求。     

百香果番茄复合果酒的工艺研究

以百香果和番茄为主要原料,对百香果番茄复合果酒的加工工艺进行了研究。结果表明,最佳的工艺参数为：百香果番茄汁液(V:V)1:2、初始糖度23.0 °Bx、酵母添加量0.25%、发酵温度22 ℃。这四种因素对百香果番茄复合果酒影响的主次顺序为百香果番茄汁V:V >酵母添加量>发酵温度>初始糖度。百香果番茄复合果酒的成品指标为：酒度10.4 °(V%),残糖4.9%,pH值为4.3,呈柠檬黄色,清澈透明无悬浮物、果香酒香浓郁、丰满爽口有新鲜感。     

海南长臀鮠人工驯养技术研究

为了更好的保护海南长臀鮠这一海南淡水土著鱼类,对其进行了人工驯养技术研究。结果表明,在池塘和网箱中,人工饲养条件下,野生的海南长臀鮠通过驯养可以主动摄食人工配合饲料,生长速度较快。     

芝麻不同生育阶段需水特性与干旱灌溉应用研究

探讨芝麻不同生育阶段需水特性与干旱灌溉水分生产效率,为芝麻高产栽培的合理灌溉和水分管理提供科学依据,2013-2018年在安徽、新疆等不同地域,选用6个芝麻主栽品种,开展芝麻不同生育阶段需水特性和灌溉水利用研究。结果表明：盆栽芝麻的全生育期蒸腾需水量平均为283.59mm,品种间差异大,形成100kg芝麻籽粒蒸腾需水量为263.56mm(175.7t/666.7m²),苗期、蕾期、初花期、盛花期、终花期、成熟期的蒸腾需水量分别为18.76mm、21.91mm、21.03mm、149.28mm、71.21mm和34.79mm,蒸腾模系数为5.89%、7.21%、6.85%、47.07%、23.93%和9.05%；盛花期最大,出苗期最小。池栽芝麻全生育期需水量531.36mm,其中株间蒸发量、蒸腾量分别占全生育期需水量的46.1%和53.9%；株间蒸发量最大的时期为苗期；蒸腾量最大的时期为花期。芝麻苗期、花期和成熟期需水模系数平均为18.65%、66.79%和14.56%。合肥基地出苗期、临泉基地花期遇旱喷灌,水分生产效率高达0.61kg/m³和0.65kg/m³；新疆精河基地按需滴灌处理比传统滴灌方式的水分生产效率提高43.28%,节水19.61%,这对水资源匮乏的新疆干旱区来说意义重大。芝麻需水量与栽培条件、品种、气温(r＝0.99)等密切相关。在芝麻不同生育阶段遇旱灌溉是提高水分生产效率和产量的关键措施之一。     

草鱼TAB1蛋白的原核重组表达及其抗体制备

为了制备草鱼重组TAB1蛋白(rCiTAB1)及其特异性抗体,首先以草鱼头肾组织c DNA为模板,PCR扩增Ci TAB1基因全长序列,并依次构建重组克隆质粒p MD19-T-Ci TAB1与重组表达质粒pET-32a-Ci TAB1。该重组表达质粒经0.5 mmol·L~(-1) IPTG,37℃诱导表达12 h后获得以包涵体形式表达的重组CiTAB1蛋白(rCiTAB1)。然后,采用3种不同方法对包涵体蛋白进行变性,发现与高浓度尿素直接变性法和洗涤后尿素变性法相比,洗涤后梯度尿素变性法处理后的蛋白纯度最好,经透析复性后得到浓度为2 mg·mL~(-1)的r Ci TAB1。最后,将rCiTAB1蛋白与白油佐剂及免疫增强剂混合,室温下混合1.5h乳化成免疫原,3次免疫新西兰大白兔制备兔抗CiTAB1抗体,经间接ELISA方法和免疫琼脂双向扩散试验测得免疫后第33天血清中特异性抗体的效价分别为1∶1 048 576和1∶16。Western blot检测到一条分子量约为72 kDa的特异性条带,表明该抗体能特异性识别r Ci TAB1蛋白。研究结果为后续深入研究CiTAB1蛋白功能提供了物质基础。     

Up-regulation of miR-181a attenuates releases of CSE-induced pro-inflammatory factors and expression of collagen IV,fibronectin and α-SMA in human bronchial epithelial cells

AIM: To investigate the effect and potential mechanism of microRNA-181a (miR-181a) on cigarette smoke extract (CSE)-induced the productions of pro-inflammatory factors and the expression of collagen IV, fibronectin and α-smooth muscle actin (α-SMA) in human bronchial epithelial cells (HBECs). METHODS: CSE-induced miR-181a expression was detected by RT-qPCR in the HBECs. After tansfected with miR-181a mimic, the releases of tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), IL-6 and transforming growth factor-β1 (TGF-β1) were measured by ELISA, the protein expression of collagen IV, fibronectin and α-SMA was determined by Western blot. The activation of NF-κB/TGF-β1/Smad3 pathway was also evaluated by Western blot. RESULTS: CSE increased the levels of TNF-α, IL-1β, IL-6 and TGF-β1 and the expression of collagen IV, fibronectin and α-SMA, and decreased the expression of miR-181a in the HBECs (P<0.05). However, transfected with miR-181a mimic partially prevented the releases of TNF-α, IL-1β, IL-6 and TGF-β1, and inhibited the expression of collagen IV, fibronectin and α-SMA (P<0.05). Additionally, the activation of NF-κB/TGF-β1/Smad3 evoked by CSE was attenuated after transfected with miR-181a mimic. CONCLUSION: Up-regulation of miR-181a prevents the releases of CSE-induced pro-inflammatory factors and expression of collagen IV, fibronectin and α-SMA in the HBECs, and its mechanism may be related to the inhibition of NF-κB/TGF-β1/Smad3 pathway.     

川黔等西南地区李子主要病虫害及防治技术

李适应性广,耐瘠薄干旱,是四川、重庆等西南山区的特色经济树种。李果实外观亮丽,脆甜爽口、营养丰富,是深受人们喜爱的特色水果之一。近年来四川、贵州、重庆地区青脆李、空心李、蜂糖李等地方品种有了迅速发展,促进了贫困山区脱贫致富和产业结构调整。但由于管理粗放和防治措施不当,致使李子生产区域病虫害发生严重,影响了果实的品质和经济效益。为了促进李子产业化发展,结合主要产区生产实际,总结出西南地区常见的病虫害及其防治措施,为李子生产提供技术参考。     

Development of a reverse-transcription loop-mediated isothermal amplification assay to detect avian influenza viruses in clinical specimens

In recent years, the avian influenza has brought not only serious economic loss to the poultry industry in China but also a serious threat to human health because of the avian influenza virus(AIV) gene recombination and reassortment. Until now, traditional RT-PCR, fluorescence RT-PCR and virus isolation identification have been developed and utilized to detect AIV, but these methods require high-level instruments and experimental conditions, not suitable for the rapid detection in field and farms. In order to develop a rapid, sensitive and practical method to detect and identify AIV subtypes, 4 specific primers to the conserved region of AIV M gene were designed and a loop-mediated isothermal amplification(RT-LAMP) method was established. Using this method, the M gene of H1–H16 subtypes of AIV were amplified in 30 min with a water bath and all 16 H subtypes of AIV were able to be visually identified in presence of fluorescein, without cross reaction with other susceptible avian viruses. In addition, the detection limit of the common H1, H5, H7, and H9 AIV subtypes with the RT-LAMP method was 0.1 PFU(plaque-forming unit), which was 10 times more sensitive than that using the routine RT-PCR. Further comparative tests found that the positivity rate of RT-LAMP on detecting clinical samples was 4.18%(14/335) comparing with 3.58%(12/335) from real-time RT-PCR. All these results suggested that the RT-LAMP method can specifically detect and identify AIV with high sensitivity and can be considered as a fast, convenient and practical method for the clinic test and epidemiological investigation of AIV.